Journal: Cell reports

Article Title: Human Glial Progenitor Cells Effectively Remyelinate the Demyelinated Adult Brain

doi: 10.1016/j.celrep.2020.107658

Figure Lengend Snippet: Human glial chimeras were maintained on either a cuprizone (CZN)-containing or control diet from 12–24 weeks of age. Twelve weeks later, at 36 weeks, the mice were killed and their resident hGPCs isolated by CD140a-based FACS, which were then subjected to RNA-seq (n = 6).

Article Snippet: AB_396285 CD140a-PE PE-conj mouse anti-human CD140a, AR1 10 μL/ 10 6 cells 556002 BD Biosci AB_396286 Secondaries Rat anti-Mouse IgM MicroBeads 20 μL/10 7 cells 130-047-301 Miltenyi AB_244358 Rat anti-Mouse IgG2a+b MicroBeads 10 μL/10 7 cells 130-047-201 Miltenyi AB_244356 AlexaFluor 568 Goat anti-Mouse IgG (H+L) 1:400 A-11031 Invitrogen AB_144696 AlexaFluor 568 Goat anti-Mouse IgG1 1:400 A-21124 Invitrogen AB_2535766 AlexaFluor 488 Goat anti-Mouse IgG (H+L) 1:400 A-11029 Invitrogen AB_2534088 AlexaFluor 488 Goat anti-Mouse IgG1 1:400 A-21121 Invitrogen AB_2535764 AlexaFluor 647 Goat anti-Mouse IgG (H+L) 1:400 A-21235 Jackson AB_2535813 AlexaFluor 568 Goat anti-Rabbit IgG (H+L) 1:400 A-11036 Invitrogen AB_2534094 AlexaFluor 488 Goat anti-Rabbit IgG (H+L) 1:400 A-11034 Invitrogen AB_2576217 AlexaFluor 568 Goat anti-Rat IgG (H+L) 1:400 A-11077 Invitrogen AB_2534121 AlexaFluor 488 Goat anti-Rat IgG (H+L) 1:400 A-11006 Invitrogen AB_2534074 Open in a separate window

Techniques: Control, Isolation, RNA Sequencing

Journal: Cell reports

Article Title: Human Glial Progenitor Cells Effectively Remyelinate the Demyelinated Adult Brain

doi: 10.1016/j.celrep.2020.107658

Figure Lengend Snippet:

Article Snippet: AB_396285 CD140a-PE PE-conj mouse anti-human CD140a, AR1 10 μL/ 10 6 cells 556002 BD Biosci AB_396286 Secondaries Rat anti-Mouse IgM MicroBeads 20 μL/10 7 cells 130-047-301 Miltenyi AB_244358 Rat anti-Mouse IgG2a+b MicroBeads 10 μL/10 7 cells 130-047-201 Miltenyi AB_244356 AlexaFluor 568 Goat anti-Mouse IgG (H+L) 1:400 A-11031 Invitrogen AB_144696 AlexaFluor 568 Goat anti-Mouse IgG1 1:400 A-21124 Invitrogen AB_2535766 AlexaFluor 488 Goat anti-Mouse IgG (H+L) 1:400 A-11029 Invitrogen AB_2534088 AlexaFluor 488 Goat anti-Mouse IgG1 1:400 A-21121 Invitrogen AB_2535764 AlexaFluor 647 Goat anti-Mouse IgG (H+L) 1:400 A-21235 Jackson AB_2535813 AlexaFluor 568 Goat anti-Rabbit IgG (H+L) 1:400 A-11036 Invitrogen AB_2534094 AlexaFluor 488 Goat anti-Rabbit IgG (H+L) 1:400 A-11034 Invitrogen AB_2576217 AlexaFluor 568 Goat anti-Rat IgG (H+L) 1:400 A-11077 Invitrogen AB_2534121 AlexaFluor 488 Goat anti-Rat IgG (H+L) 1:400 A-11006 Invitrogen AB_2534074 Open in a separate window

Techniques: In Vivo, In Vitro

Journal: Cell reports

Article Title: Human Glial Progenitor Cells Effectively Remyelinate the Demyelinated Adult Brain

doi: 10.1016/j.celrep.2020.107658

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: AB_396285 CD140a-PE PE-conj mouse anti-human CD140a, AR1 10 μL/ 10 6 cells 556002 BD Biosci AB_396286 Secondaries Rat anti-Mouse IgM MicroBeads 20 μL/10 7 cells 130-047-301 Miltenyi AB_244358 Rat anti-Mouse IgG2a+b MicroBeads 10 μL/10 7 cells 130-047-201 Miltenyi AB_244356 AlexaFluor 568 Goat anti-Mouse IgG (H+L) 1:400 A-11031 Invitrogen AB_144696 AlexaFluor 568 Goat anti-Mouse IgG1 1:400 A-21124 Invitrogen AB_2535766 AlexaFluor 488 Goat anti-Mouse IgG (H+L) 1:400 A-11029 Invitrogen AB_2534088 AlexaFluor 488 Goat anti-Mouse IgG1 1:400 A-21121 Invitrogen AB_2535764 AlexaFluor 647 Goat anti-Mouse IgG (H+L) 1:400 A-21235 Jackson AB_2535813 AlexaFluor 568 Goat anti-Rabbit IgG (H+L) 1:400 A-11036 Invitrogen AB_2534094 AlexaFluor 488 Goat anti-Rabbit IgG (H+L) 1:400 A-11034 Invitrogen AB_2576217 AlexaFluor 568 Goat anti-Rat IgG (H+L) 1:400 A-11077 Invitrogen AB_2534121 AlexaFluor 488 Goat anti-Rat IgG (H+L) 1:400 A-11006 Invitrogen AB_2534074 Open in a separate window

Techniques: Marker, In Vitro, Recombinant, Clinical Proteomics, Software, Microscopy, Lysis

Journal: Genes

Article Title: Myelin-Specific microRNA-23a/b Cluster Deletion Inhibits Myelination in the Central Nervous System during Postnatal Growth and Aging

doi: 10.3390/genes15040402

Figure Lengend Snippet: Immunostaining of oligodendrocytes and their progenitor cells in brain sections. Control (Cont) and myelin-specific miR-23a/b cluster knockout mice (KO); n = 7 per group. ( A ) Immunostaining images of the brain corpus callosum stained with platelet-derived growth factor α (PDGFα), CC1, and OLIG2 of 10-week-old (10 W) and 12-month-old (12 M) Cont and KO mice. ( B ) Number per unit area of mature oligodendrocytes showing double-positivity for CC1 and OLIG2. ( C ) Number per unit area of oligodendrocyte progenitors showing double-positivity for PDGFRα and OLIG2.

Article Snippet: The sections were then stained with the following primary antibodies: rabbit anti-Olig-2 (1:500, AB9610; Sigma-Aldrich, St. Louis, MO, USA), goat anti-platelet-derived growth factor receptor α (PDGFRα; 1:200, AF1062; R&D Systems, Minneapolis, MN, USA), mouse anti-CC1 (1:100, OP80; Sigma-Aldrich), and rat anti-myelin basic protein (MBP; 1:250, ab7349; Abcam, Cambridge, UK).

Techniques: Immunostaining, Control, Knock-Out, Staining, Derivative Assay

Journal: Genes

Article Title: Myelin-Specific microRNA-23a/b Cluster Deletion Inhibits Myelination in the Central Nervous System during Postnatal Growth and Aging

doi: 10.3390/genes15040402

Figure Lengend Snippet: Immunostaining of oligodendrocytes and their progenitor cells in brain sections. Control (Cont) and myelin-specific miR-23a/b cluster knockout mice (KO); n = 7 per group. ( A ) Immunostaining images of the spinal cord white matter stained with platelet-derived growth factor α (PDGFα), CC1, and OLIG2 of 10-week-old (10 W) and 12-month-old (12 M) Cont and KO mice. ( B ) Number per unit area of mature oligodendrocytes showing double-positivity for CC1 and OLIG2. ( C ) Number per unit area of oligodendrocyte progenitors showing double-positivity for PDGFRα and OLIG2.

Article Snippet: The sections were then stained with the following primary antibodies: rabbit anti-Olig-2 (1:500, AB9610; Sigma-Aldrich, St. Louis, MO, USA), goat anti-platelet-derived growth factor receptor α (PDGFRα; 1:200, AF1062; R&D Systems, Minneapolis, MN, USA), mouse anti-CC1 (1:100, OP80; Sigma-Aldrich), and rat anti-myelin basic protein (MBP; 1:250, ab7349; Abcam, Cambridge, UK).

Techniques: Immunostaining, Control, Knock-Out, Staining, Derivative Assay